cd14 microbeads (Miltenyi Biotec)
Structured Review

Cd14 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4691 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd14+microbeads+human/CD14+MicroBeads%2C+human/pmc13650181-239-26-28
Average 98 stars, based on 4691 article reviews
Images
1) Product Images from "A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma"
Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
Journal: Smart Medicine
doi: 10.1002/smmd.70058
Figure Legend Snippet: Rosasina drives the pro‐inflammatory polarization of primary human macrophages. Primary human monocyte‐derived macrophages were generated by differentiation in M‐CSF and subsequently treated with increasing concentrations of the ROS‐inducing agent Rosasina (1, 10, and 100 nM). Following treatment, macrophage polarization was assessed by multiparameter flow cytometry using established M1‐and M2‐associated surface markers. Cells were gated on live, single CD45 + /CD14 + macrophages prior to analysis. (A–F) Representative overlaid histograms and contour plots showing expression of M1‐associated markers. IFN‐γ (A, B) and MHC‐II (D, E) across treatment conditions. Quantification of M1 marker expression, presented as percentage of positive cells (C, F). (G–L) Representative overlaid histograms and contour plots showing the expression of M2‐associated markers. CD206 (G, H) and CD163) (J, K). Quantification of M2 marker expression (I, L) across treatment groups. (M) Cell viability following Rosasina exposure assessed by MTT assay to evaluate potential cytotoxic effects of treatment. Data represent independent experiments performed with primary human donors ( n = 4/group) in biological replicates. Quantified data are presented as mean ± SD. Statistical analysis was performed using ANOVA, with significance indicated as p < 0.05 compared with mock‐treated controls.
Techniques Used: Derivative Assay, Generated, Flow Cytometry, Analysis, Expressing, Marker, MTT Assay
Related Articles
Isolation:Article Title: Establishment of a high-content compatible platform to assess effects of monocyte-derived factors on neural stem cell proliferation and differentiation. Article Snippet: .. Monocytes were then isolated from thawed PBMCs via positive magnetic activated cell sorting (MACS) using Article Title: The gut microbiota protein BOC1 exhibits immune checkpoint inhibitor-like activity by inhibiting myeloid-derived suppressor cell differentiation Article Snippet: Finally, PBMCs were counted with a LUNA-FLTM fluorescence cell counter using Acridine Orange/Propidium Iodide Stain for viability assessment (Logos Biosystems by Aligned Genetics, Inc., Villeneuve d’Ascq, France). .. CD14 + and CD3 + cells were purified by positive selection using Article Title: Rejuvenation of peripheral immune cells attenuates Alzheimer's disease-like pathologies and behavioral deficits in a mouse model. Article Snippet: .. PBMCs were isolated by density gradient centrifugation using Ficoll-Hypaque (TBD Science, Tianjin, China), mononuclear sections were collected, washed with phosphate-buffered saline (PBS) three times, and then used for monocytes isolation using Article Title: EB1010, a putative Christensenellaceae-derived microbial peptide, exhibits anti-inflammatory activity and therapeutic potential in ulcerative colitis Article Snippet: .. The CD14-negative fraction was obtained following CD14+ cells depletion using Article Title: Rejuvenation of peripheral immune cells attenuates Alzheimer’s disease-like pathologies and behavioral deficits in a mouse model Article Snippet: .. PBMCs were isolated by density gradient centrifugation using Ficoll-Hypaque (TBD Science, Tianjin, China), mononuclear sections were collected, washed with phosphate-buffered saline (PBS) three times, and then used for monocytes isolation using Article Title: Reprogramming the melanoma and immunosuppressive myeloid cells with esomeprazole-loaded PLGA nanoparticles Article Snippet: The analysis of the cell cycle was performed by determining the DNA content distribution after propidium iodide staining, using a Cytoflex S and Kaluza software (both Beckman Coulter) according to. .. Monocytes were isolated from PBMC using the FACS:Article Title: Establishment of a high-content compatible platform to assess effects of monocyte-derived factors on neural stem cell proliferation and differentiation. Article Snippet: .. Monocytes were then isolated from thawed PBMCs via positive magnetic activated cell sorting (MACS) using Magnetic Cell Separation:Article Title: Establishment of a high-content compatible platform to assess effects of monocyte-derived factors on neural stem cell proliferation and differentiation. Article Snippet: .. Monocytes were then isolated from thawed PBMCs via positive magnetic activated cell sorting (MACS) using Concentration Assay:Article Title: Establishment of a high-content compatible platform to assess effects of monocyte-derived factors on neural stem cell proliferation and differentiation. Article Snippet: .. Monocytes were then isolated from thawed PBMCs via positive magnetic activated cell sorting (MACS) using Article Title: Reprogramming the melanoma and immunosuppressive myeloid cells with esomeprazole-loaded PLGA nanoparticles Article Snippet: The analysis of the cell cycle was performed by determining the DNA content distribution after propidium iodide staining, using a Cytoflex S and Kaluza software (both Beckman Coulter) according to. .. Monocytes were isolated from PBMC using the Purification:Article Title: The gut microbiota protein BOC1 exhibits immune checkpoint inhibitor-like activity by inhibiting myeloid-derived suppressor cell differentiation Article Snippet: Finally, PBMCs were counted with a LUNA-FLTM fluorescence cell counter using Acridine Orange/Propidium Iodide Stain for viability assessment (Logos Biosystems by Aligned Genetics, Inc., Villeneuve d’Ascq, France). .. CD14 + and CD3 + cells were purified by positive selection using Selection:Article Title: The gut microbiota protein BOC1 exhibits immune checkpoint inhibitor-like activity by inhibiting myeloid-derived suppressor cell differentiation Article Snippet: Finally, PBMCs were counted with a LUNA-FLTM fluorescence cell counter using Acridine Orange/Propidium Iodide Stain for viability assessment (Logos Biosystems by Aligned Genetics, Inc., Villeneuve d’Ascq, France). .. CD14 + and CD3 + cells were purified by positive selection using Gradient Centrifugation:Article Title: Rejuvenation of peripheral immune cells attenuates Alzheimer's disease-like pathologies and behavioral deficits in a mouse model. Article Snippet: .. PBMCs were isolated by density gradient centrifugation using Ficoll-Hypaque (TBD Science, Tianjin, China), mononuclear sections were collected, washed with phosphate-buffered saline (PBS) three times, and then used for monocytes isolation using Article Title: Rejuvenation of peripheral immune cells attenuates Alzheimer’s disease-like pathologies and behavioral deficits in a mouse model Article Snippet: .. PBMCs were isolated by density gradient centrifugation using Ficoll-Hypaque (TBD Science, Tianjin, China), mononuclear sections were collected, washed with phosphate-buffered saline (PBS) three times, and then used for monocytes isolation using Saline:Article Title: Rejuvenation of peripheral immune cells attenuates Alzheimer's disease-like pathologies and behavioral deficits in a mouse model. Article Snippet: .. PBMCs were isolated by density gradient centrifugation using Ficoll-Hypaque (TBD Science, Tianjin, China), mononuclear sections were collected, washed with phosphate-buffered saline (PBS) three times, and then used for monocytes isolation using Article Title: Rejuvenation of peripheral immune cells attenuates Alzheimer’s disease-like pathologies and behavioral deficits in a mouse model Article Snippet: .. PBMCs were isolated by density gradient centrifugation using Ficoll-Hypaque (TBD Science, Tianjin, China), mononuclear sections were collected, washed with phosphate-buffered saline (PBS) three times, and then used for monocytes isolation using Incubation:Article Title: Impact of cooperative or competitive dynamics between the yeast Saccharomyces cerevisiae and lactobacilli on the immune response of the host. Article Snippet: .. 100 ml of Article Title: Impact of cooperative or competitive dynamics between the yeast Saccharomyces cerevisiae and lactobacilli on the immune response of the host Article Snippet: .. 100 μl of Cell Culture:Article Title: Reprogramming the melanoma and immunosuppressive myeloid cells with esomeprazole-loaded PLGA nanoparticles Article Snippet: The analysis of the cell cycle was performed by determining the DNA content distribution after propidium iodide staining, using a Cytoflex S and Kaluza software (both Beckman Coulter) according to. .. Monocytes were isolated from PBMC using the |